Evaluation of standard curves in the graded hemagglutination-inhibition method applied to quantitation of human immunoglobulins.

Preparation of an Inactivated Egg Drop Syndrome Antigen for Using in Hemagglutination Inhibition Test.

To prepare hemagglutination inhibition (HI) antigen the egg drop syndrome virus (EDSV) was propagated in 10-day-old embryonated duck eggs. The virus antigen was inactivated with two methods including heating (65°C) and adding 0.5% formaldehyde by considering low destroying effect on hemagglutination (HA) and HI titers. The EDSV HI titers of 310 sera and 100 yolks obtained from 23 chicken farms ...

Simultaneous Quantitation of Theophylline and Guaifenesin in Syrup by HPLC, Derivative and Derivative Ratio Spectrophotometry for Quality Control Purposes

The aim of the present work was to develop a simple and rapid method for determination of theophylline (THP) and guaifenesin (GU) in syrup without involving any preparation operations like separation or masking. A HPLC and two spectrophotometric methods based on the derivation of the main spectra are described for the determination of THP and GU in combined pharmaceutical syrup form. The first ...

Serological prevalence of avian H9N2 influenza virus in dogs by hemagglutination inhibition assay in Kerman, southeast of Iran

Influenza is a highly contagious zoonotic disease in the world. Avian H9N2 influenza virus is a significant pandemic pathogen widely distributed throughout the world. Pet ownership has been documented as a risk factor for infection transmission to human. Considering major public health concern, the prevalence of antibodies against avian H9N2 influenza virus was evaluated in 170 serum samples of...

Quantitation of serum IgM by graded hemagglutination inhibition.

Evaluation of Immune Status to Measles in Vaccinated Population in Tehran, by Enzyme-linked Immunosorbent Assay and the Hemagglutination Inhibition Techniques (1386-1387)

Background and Aims: Measles remains one of the leading causes of childhood morbidity and mortality in developing countries and is still a major public health concern in developed countries. Although live attenuated vaccine is used throughout the world, out breaks of disease still occur in many countries including Iran. Methods: The present study was performed to evaluate the immune status aga...